Expression of the transcription factor HIF-1α – essential for a cell’s response to low oxygen conditions – is inhibited by drugs that stabilize or depolymerize the microtubule cytoskeleton. Carbonaro et al. find that HIF-1α mRNA is usually transported along microtubules and translated into protein. But disrupting microtubules with drugs such as taxol or nocodazole shifts HIF-1α mRNA into cytoplasmic structures called P-bodies, where its translation is repressed by miRNAs.
Download our Journal Club pack to aid your discussion of Carbonaro et al.’s study, and its implications for both HIF-1α regulation and clinical oncology. The pack includes a complete pdf of the paper, a PowerPoint file of Carbonaro et al.’s figures, supplementary videos, and the latest biosights video podcast, which features an interview with two of the study’s authors, Marisa Carbonaro and Paraskevi Giannakakou (Weill Cornell Medical College, New York, NY).
Download Complete Journal Club Pack (70.7MB zip file)