At the start of mitosis, the nuclear envelope and nuclear pore complexes break down and disperse into the endoplasmic reticulum and cytoplasm. Lu et al. use rapid, 3D live cell imaging to reveal that, in contrast to previous models, the nuclear envelope reforms directly from ER cisternae after mitosis and that this happens before nuclear pore complexes start to reassemble.
Download our Journal Club pack to aid your discussion of Lu et al.’s study, and its implications for post-mitotic nuclear pore complex and nuclear envelope assembly. The pack includes a complete pdf of the paper, a PowerPoint file of Lu et al.’s figures, supplementary videos, and the latest biosights video podcast, which features an interview with two of the study’s authors, Lei Lu (Nanyang Technological University, Singapore) and Tomas Kirchhausen (Harvard Medical School, Boston, MA).