The actin cytoskeleton has been proposed to regulate exocytosis in many different ways. Nightingale et al. use a two color, live cell imaging assay to reveal two contrasting functions of actin in distinct stages of Weibel-Palade body secretion: actin-based anchors inhibit the fusion of these secretory granules with the plasma membrane but, post-fusion, a contractile actin ring squeezes granule content out of the cell.
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